Olesoxime protects embryonic cortical neurons from camptothecin intoxication by a mechanism distinct from BDNF.

ABSTRACT:

Background and purpose

Olesoxime is a small cholesterol-oxime promoting rat embryonic motor neurons survival in the absence of trophic factors. Because olesoxime can substitute for neurotrophic factors in many situations, and to gain further understanding of its mechanism of action, we wondered if it could prevent neuronal death induced by camptothecin (CPT) and compared its effects with those of brain-derived neurotrophic factor (BDNF).

Experimental approach

E17 rat embryonic cortical neurons were treated with olesoxime, BDNF or vehicle and intoxicated with CPT. Caspase-dependent and caspase-independent death pathways along with pro-survival pathways activation were explored.

Key results

As previously reported for BDNF, olesoxime dose-dependently delayed CPT-induced

SUBMITTER: Gouarne C 

PROVIDER: S-EPMC3623066 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature