Synthetically tractable click hydrogels for three-dimensional cell culture formed using tetrazine-norbornene chemistry.

ABSTRACT: The implementation of bio-orthogonal click chemistries is a topic of growing importance in the field of biomaterials, as it is enabling the development of increasingly complex hydrogel materials capable of providing dynamic, cell-instructive microenvironments. Here, we introduce the tetrazine-norbornene inverse electron demand Diels-Alder reaction as a new cross-linking chemistry for the formation of cell laden hydrogels. The fast reaction rate and irreversible nature of this click reaction allowed for hydrogel formation within minutes when a multifunctional PEG-tetrazine macromer was reacted with a dinorbornene peptide. In addition, the cytocompatibility of the polymerization led to high postencapsulation viability of human mesenchymal stem cells, and the specificity of the tetrazine-norb

SUBMITTER: Alge DL 

PROVIDER: S-EPMC3623454 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature