Distinguishing core and holoenzyme mechanisms of transcription termination by RNA polymerase III.

ABSTRACT: Transcription termination by RNA polymerase (Pol) III serves multiple purposes; it delimits interference with downstream genes, forms 3' oligo(U) binding sites for the posttranscriptional processing factor, La protein, and resets the polymerase complex for reinitiation. Although an interplay of several Pol III subunits is known to collectively control these activities, how they affect molecular function of the active center during termination is incompletely understood. We have approached this using immobilized Pol III-nucleic acid scaffolds to examine the two major components of termination, transcription pausing and RNA release. This allowed us to distinguish two mechanisms of termination by isolated Saccharomyces cerevisiae Pol III. A core mechanism can operate in the absence of C53/37

SUBMITTER: Arimbasseri AG 

PROVIDER: S-EPMC3624256 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature