Multiparametric analysis of CLASP-interacting protein functions during interphase microtubule dynamics.

ABSTRACT: The microtubule (MT) plus-end tracking protein (+TIP) CLASP mediates dynamic cellular behaviors and interacts with numerous cytoplasmic proteins. While the influence of some CLASP interactors on MT behavior is known, a comprehensive survey of the proteins in the CLASP interactome as MT regulators is missing. Ultimately, we are interested in understanding how CLASP collaborates with functionally linked proteins to regulate MT dynamics. Here, we utilize multiparametric analysis of time-lapse MT +TIP imaging data acquired in Drosophila melanogaster S2R+ cells to assess the effects on individual microtubule dynamics for RNA interference-mediated depletion of 48 gene products previously identified to be in vivo genetic CLASP interactors. While our analysis corroborates previously described func

SUBMITTER: Long JB 

PROVIDER: S-EPMC3624261 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature