Mapping of sequences in Pseudorabies virus pUL34 that are required for formation and function of the nuclear egress complex.

ABSTRACT: The nuclear egress complex (NEC) is required for efficient translocation of newly synthesized herpesvirus nucleocapsids from the nucleus to the cytosol. It consists of the type II membrane protein pUL34 which interacts with pUL31 at the inner nuclear membrane (INM). To map regions within pUL34 required for nuclear membrane targeting and pUL31 interaction, we constructed deletion/substitution mutations. Previously, we showed that 50 C-terminal amino acids (aa) of pseudorabies virus (PrV) pUL34, including the transmembrane domain, could be functionally replaced by cellular lamina-associated polypeptide 2β (Lap2β) sequences. In contrast, replacement of the C-terminal 100 aa abrogated complementation but not pUL31 interaction. To further delineate essential sequences within this region, C-term

SUBMITTER: Paßvogel L 

PROVIDER: S-EPMC3624375 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature