Effects of H2 and formate on growth yield and regulation of methanogenesis in Methanococcus maripaludis.

ABSTRACT: Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to formate starvation have not been reported. Here we report that during continuous culture of Methanococcus maripaludis under defined nutrient conditions, growth yields relative to methane production decreased markedly with either H2 excess or formate excess. Analysis of the growth yields of several mutants suggests that this phenomenon occurs independently of the storage of intracellular carbon or a transcriptional response to methanogenesis. Using microarray analysis, we found t

SUBMITTER: Costa KC 

PROVIDER: S-EPMC3624518 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature