Restricted heterochromatin formation links NFATc2 repressor activity with growth promotion in pancreatic cancer.

ABSTRACT:

Background & aims

Transcriptional silencing of the p15(INK4b) tumor suppressor pathway overcomes cellular protection against unrestrained proliferation in cancer. Here we show a novel pathway involving the oncogenic transcription factor nuclear factor of activated T cells (NFAT) c2 targeting a p15(INK4b)-mediated failsafe mechanism to promote pancreatic cancer tumor growth.

Methods

Immunohistochemistry, real-time polymerase chain reaction, immunoblotting, and immunofluorescence microscopy were used for expression studies. Cancer growth was assessed in vitro by [(3)H]thymidine incorporation, colony formation assays, and in vivo using xenograft tumor models. Protein-protein interactions, promoter regulation, and local histone modifications were analyzed by immunoprecipitation,

SUBMITTER: Baumgart S 

PROVIDER: S-EPMC3626431 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature