Genome-wide gene expression analysis of anguillid herpesvirus 1.

ABSTRACT:

Background

Whereas temporal gene expression in mammalian herpesviruses has been studied extensively, little is known about gene expression in fish herpesviruses. Here we report a genome-wide transcription analysis of a fish herpesvirus, anguillid herpesvirus 1, in cell culture, studied during the first 6 hours of infection using reverse transcription quantitative PCR.

Results

Four immediate-early genes - open reading frames 1, 6A, 127 and 131 - were identified on the basis of expression in the presence of a protein synthesis inhibitor and unique expression profiles during infection in the absence of inhibitor. All of these genes are located within or near the terminal direct repeats. The remaining 122 open reading frames were clustered into groups on the basis of transcripti

SUBMITTER: van Beurden SJ 

PROVIDER: S-EPMC3626852 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature