Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.

ABSTRACT: Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone NHEJ can be efficiently suppressed by 'nickases' that produce a single-strand break rather than a double-strand break. Highly specific nickases have been produced by engineering of homing endonucleases and more recently by modifying zinc finger nucleases (ZFNs) composed of a zinc finger array and the catalytic domain of the restriction endonuclease FokI. These ZF-nickases work as heterodimers in which one subunit has a catalytically inactive FokI domain. We present two differ

SUBMITTER: Gabsalilow L 

PROVIDER: S-EPMC3627573 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature