Ontology highlight
ABSTRACT:
SUBMITTER: Dalgarno PA
PROVIDER: S-EPMC3627600 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Dalgarno Paul A PA Bordello Jorge J Morris Rhodri R St-Pierre Patrick P Dubé Audrey A Samuel Ifor D W ID Lafontaine Daniel A DA Penedo J Carlos JC
Nucleic acids research 20130227 7
To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure. Here, we introduce a novel strategy that combines single-molecule Förster resonance energy transfer (FRET) and chemical denaturation to observe and manipulate RNA dynamics. We demonstrate that the competing interplay between metal ions and denaturant agents provides a platform to extract information that otherwise will remain hidden ...[more]