Conserved structural domains in FoxD4L1, a neural forkhead box transcription factor, are required to repress or activate target genes.

ABSTRACT: FoxD4L1 is a forkhead transcription factor that expands the neural ectoderm by down-regulating genes that promote the onset of neural differentiation and up-regulating genes that maintain proliferative neural precursors in an immature state. We previously demonstrated that binding of Grg4 to an Eh-1 motif enhances the ability of FoxD4L1 to down-regulate target neural genes but does not account for all of its repressive activity. Herein we analyzed the protein sequence for additional interaction motifs and secondary structure. Eight conserved motifs were identified in the C-terminal region of fish and frog proteins. Extending the analysis to mammals identified a high scoring motif downstream of the Eh-1 domain that contains a tryptophan residue implicated in protein-protein interactions. In

SUBMITTER: Klein SL 

PROVIDER: S-EPMC3627651 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature