STIM1 restores coronary endothelial function in type 1 diabetic mice.

ABSTRACT:

Rationale

The endoplasmic reticulum (ER) is a major intracellular Ca(2+) store in endothelial cells (ECs). The Ca(2+) concentration in the ER greatly contributes to the generation of Ca(2+) signals that regulate endothelial functions. Many proteins, including stromal interaction molecule 1/2 (STIM1/2), Orai1/2/3, and sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase 3 (SERCA3), are involved in the ER Ca(2+) refilling after store depletion in ECs.

Objective

This study is designed to examine the role of Ca(2+) in the ER in coronary endothelial dysfunction in diabetes.

Methods and results

Mouse coronary ECs (MCECs) isolated from diabetic mice exhibited (1) a significant decrease in the Ca(2+) mobilization from the ER when the cells were treated by SERCA inhibitor, and (2)

SUBMITTER: Estrada IA 

PROVIDER: S-EPMC3627729 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature