Identification of human monoclonal antibodies specific for human SOD1 recognizing distinct epitopes and forms of SOD1.

ABSTRACT: Mutations in the gene encoding human SOD1 (hSOD1) can cause amyotrophic lateral sclerosis (ALS) yet the mechanism by which mutant SOD1 can induce ALS is not fully understood. There is currently no cure for ALS or treatment that significantly reduces symptoms or progression. To develop tools to understand the protein conformations present in mutant SOD1-induced ALS and as possible immunotherapy, we isolated and characterized eleven unique human monoclonal antibodies specific for hSOD1. Among these, five recognized distinct linear epitopes on hSOD1 that were not available in the properly-folded protein but were available on forms of protein with some degree of misfolding. The other six antibodies recognized conformation-dependent epitopes that were present in the properly-folded protein with

SUBMITTER: Broering TJ 

PROVIDER: S-EPMC3629177 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature