Reduction of ribosome level triggers flocculation of fission yeast cells.

ABSTRACT: Deletion of ribosomal protein L32 genes resulted in a nonsexual flocculation of fission yeast. Nonsexual flocculation also occurred when two other ribosomal protein genes, rpl21-2 and rpl9-2, were deleted. However, deletion of two nonribosomal protein genes, mpg and fbp, did not cause flocculation. Overall transcript levels of rpl32 in rpl32-1Δ and rpl32-2Δ cells were reduced by 35.9% and 46.9%, respectively, and overall ribosome levels in rpl32-1Δ and rpl32-2Δ cells dropped 31.1% and 27.8%, respectively, compared to wild-type cells. Interestingly, ribosome protein expression levels and ribosome levels were also reduced greatly in sexually flocculating diploid YHL6381/WT (h⁺/h⁻) cells compared to a mixture of YHL6381 (h⁺) and WT (h⁻) nonflocculating haploid cells. Transcriptome analysis in

SUBMITTER: Li R 

PROVIDER: S-EPMC3629774 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature