Emission spectra profiling of fluorescent proteins in living plant cells.

ABSTRACT:

Background

Fluorescence imaging at high spectral resolution allows the simultaneous recording of multiple fluorophores without switching optical filters, which is especially useful for time-lapse analysis of living cells. The collected emission spectra can be used to distinguish fluorophores by a computation analysis called linear unmixing. The availability of accurate reference spectra for different fluorophores is crucial for this type of analysis. The reference spectra used by plant cell biologists are in most cases derived from the analysis of fluorescent proteins in solution or produced in animal cells, although these spectra are influenced by both the cellular environment and the components of the optical system. For instance, plant cells contain various autofluorescent compo

SUBMITTER: Mylle E 

PROVIDER: S-EPMC3630006 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature