Comprehensive assignment of roles for Salmonella typhimurium genes in intestinal colonization of food-producing animals.

ABSTRACT: Chickens, pigs, and cattle are key reservoirs of Salmonella enterica, a foodborne pathogen of worldwide importance. Though a decade has elapsed since publication of the first Salmonella genome, thousands of genes remain of hypothetical or unknown function, and the basis of colonization of reservoir hosts is ill-defined. Moreover, previous surveys of the role of Salmonella genes in vivo have focused on systemic virulence in murine typhoid models, and the genetic basis of intestinal persistence and thus zoonotic transmission have received little study. We therefore screened pools of random insertion mutants of S. enterica serovar Typhimurium in chickens, pigs, and cattle by transposon-directed insertion-site sequencing (TraDIS). The identity and relative fitness in each host of 7,702 mutants

SUBMITTER: Chaudhuri RR 

PROVIDER: S-EPMC3630085 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature