Closing the gap between single molecule and bulk FRET analysis of nucleosomes.

ABSTRACT: Nucleosome structure and stability affect genetic accessibility by altering the local chromatin morphology. Recent FRET experiments on nucleosomes have given valuable insight into the structural transformations they can adopt. Yet, even if performed under seemingly identical conditions, experiments performed in bulk and at the single molecule level have given mixed answers due to the limitations of each technique. To compare such experiments, however, they must be performed under identical conditions. Here we develop an experimental framework that overcomes the conventional limitations of each method: single molecule FRET experiments are carried out at bulk concentrations by adding unlabeled nucleosomes, while bulk FRET experiments are performed in microplates at concentrations near those

SUBMITTER: Gansen A 

PROVIDER: S-EPMC3630217 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature