Reflectance speckle of retinal nerve fiber layer reveals axonal activity.

ABSTRACT:

Purpose

This study investigated the retinal nerve fiber layer (RNFL) reflectance speckle and tested the hypothesis that temporal change of RNFL speckle reveals axonal dynamic activity.

Methods

RNFL reflectance speckle of isolated rat retinas was studied with monochromatic illumination. A series of reflectance images was collected every 5 seconds for approximately 15 minutes. Correlation coefficients (CC) of selected areas between a reference and subsequent images were calculated and plotted as a function of the time intervals between images. An exponential function fit to the time course was used to evaluate temporal change of speckle pattern. To relate temporal change of speckle to axonal activity, in vitro living retina perfused at a normal (34°C) and a lower (24°C) temper

SUBMITTER: Huang XR 

PROVIDER: S-EPMC3630816 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature