Structure of a complex phosphoglycan epitope from gp72 of Trypanosoma cruzi.

ABSTRACT: The parasitic protozoan organism Trypanosoma cruzi is the causative agent of Chagas disease. The insect vector-dwelling epimastigote form of the organism expresses a low abundance glycoprotein associated with the flagellum adhesion zone, called gp72. The gp72 glycoprotein was first identified with an anti-carbohydrate IgG3 monoclonal antibody called WIC29.26 and has been shown to have an unusual sugar composition. Here, we describe a new way to isolate the WIC29.26 carbohydrate epitope of gp72. Using (1)H NMR and mass spectrometry before and after derivatization, we provide an almost complete primary chemical structure for the epitope, which is that of a complex phosphosaccharide: Galfβ1-4Rhapα1-2Fucpα1-4(Galpβ1-3)(Galpα1-2)Xylpβ1-4Xylpβ1-3(Xylpβ1-2Galpα1-4(Galpβ1-3)(Rhapα1-2)Fucpα1-4)GlcN

SUBMITTER: Allen S 

PROVIDER: S-EPMC3630849 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature