Meiosis-specific failure of cell cycle progression in fission yeast by mutation of a conserved beta-tubulin residue.

ABSTRACT: The microtubule cytoskeleton is involved in regulation of cell morphology, differentiation, and cell cycle progression. Precisely controlled dynamic properties are required for these microtubule functions. To better understand how tubulin's dynamics are embedded in its primary sequence, we investigated in vivo the consequences of altering a single, highly conserved residue in beta-tubulin that lies at the interface between two structural domains. The residue differs between the cold-adapted Antarctic fish and temperate animals in a manner that suggests a role in microtubule stability. Fungi, like the Antarctic fish, have a phenylalanine in this position, whereas essentially all other animals have tyrosine. We mutated the corresponding residue in fission yeast to tyrosine. Temperature effec

SUBMITTER: Paluh JL 

PROVIDER: S-EPMC363098 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature