Transcription of Drosophila troponin I gene is regulated by two conserved, functionally identical, synergistic elements.

ABSTRACT: The Drosophila wings-up A gene encodes Troponin I. Two regions, located upstream of the transcription initiation site (upstream regulatory element) and in the first intron (intron regulatory element), regulate gene expression in specific developmental and muscle type domains. Based on LacZ reporter expression in transgenic lines, upstream regulatory element and intron regulatory element yield identical expression patterns. Both elements are required for full expression levels in vivo as indicated by quantitative reverse transcription-polymerase chain reaction assays. Three myocyte enhancer factor-2 binding sites have been functionally characterized in each regulatory element. Using exon specific probes, we show that transvection is based on transcriptional changes in the homologous chromos

SUBMITTER: Marin MC 

PROVIDER: S-EPMC363105 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature