Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae.

ABSTRACT: The budding yeast Saccharomyces cerevisiae responds to depletion of iron in the environment by activating Aft1p, the major iron-dependent transcription factor, and by transcribing systems involved in the uptake of iron. Here, we have studied the transcriptional response to iron deprivation and have identified new Aft1p target genes. We find that other metabolic pathways are regulated by iron: biotin uptake and biosynthesis, nitrogen assimilation, and purine biosynthesis. Two enzymes active in these pathways, biotin synthase and glutamate synthase, require an iron-sulfur cluster for activity. Iron deprivation activates transcription of the biotin importer and simultaneously represses transcription of the entire biotin biosynthetic pathway. Multiple genes involved in nitrogen assimilation an

SUBMITTER: Shakoury-Elizeh M 

PROVIDER: S-EPMC363115 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature