Flowering time control in ornamental gloxinia (Sinningia speciosa) by manipulation of miR159 expression.

ABSTRACT:

Background and aims

Gloxinia (Sinningia speciosa) is a popular commercial plant for its attractive and colourful flowers. However, the genetic mechanism of flowering time regulation in gloxinia is largely unknown. Recent studies on model plants have elucidated that miR159 acts as a negative regulator of floral transition in short-day photoperiods. The aim of this study was to investigate whether genetic modification of miR159 expression can offer an effective approach for regulation of flowering characteristics in gloxinia.

Methods

Transgenic gloxinia plants were generated that over-express or suppress miR159 by means an efficient Agrobacterium-mediated transformation system in order to study the effect of miR159 on flowering time. In addition, the full-length cDNA of gloxin

SUBMITTER: Li X 

PROVIDER: S-EPMC3631324 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature