DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity.

ABSTRACT:

Background and aims

The germination test currently represents the most used method to assess seed viability in germplasm banks, despite the difficulties caused by the occurrence of seed dormancy. Furthermore, seed longevity can vary considerably across species and populations from different environments, and studies related to the eco-physiological processes underlying such variations are still limited in their depth. The aim of the present work was the identification of reliable molecular markers that might help in monitoring seed deterioration.

Methods

Dry seeds were subjected to artificial ageing and collected at different time points for molecular/biochemical analyses. DNA damage was measured using the RAPD (random amplified polymorphic DNA) approach while the seed antio

SUBMITTER: Dona M 

PROVIDER: S-EPMC3631342 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature