Assignment of 1H, 13C and 15N backbone resonances of Escherichia coli LpxC bound to L-161,240.

ABSTRACT: The UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase LpxC catalyzes the committed reaction of lipid A biosynthesis, an essential pathway in Gram-negative bacteria. We report the backbone resonance assignments of the 34 kDa LpxC from Escherichia coli in complex with the antibiotic L-161,240 using multidimensional, multinuclear NMR experiments. The (1)H chemical shifts of complexed L-161,240 are also determined.

SUBMITTER: Barb AW 

PROVIDER: S-EPMC3631426 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature