In vitro hypertrophy and calcification of human fracture haematoma-derived cells in chondrogenic differentiation.

ABSTRACT:

Purpose

The haematoma at a fracture site plays an important role in fracture healing. Previously, we demonstrated that a fracture haematoma contains multilineage mesenchymal progenitor cells. We postulated that the haematoma provided a source of chondrogenic cells for endochondral ossification during fracture healing and preservation of the cells contributed to biological fracture healing. In this study, we investigated whether haematoma-derived cells (HCs) could differentiate into hypertrophic chondrocytes and finally induce calcification of the extracellular matrix in vitro.

Methods

Fracture haematomas were obtained from four patients. HCs were cultured for five weeks under conditions that induce chondrogenic differentiation, followed by two weeks of hypertrophic induction

SUBMITTER: Koga T 

PROVIDER: S-EPMC3631505 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature