A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium.

ABSTRACT: Salmonella Typhi and Typhimurium diverged only ∼50 000 years ago, yet have very different host ranges and pathogenicity. Despite the availability of multiple whole-genome sequences, the genetic differences that have driven these changes in phenotype are only beginning to be understood. In this study, we use transposon-directed insertion-site sequencing to probe differences in gene requirements for competitive growth in rich media between these two closely related serovars. We identify a conserved core of 281 genes that are required for growth in both serovars, 228 of which are essential in Escherichia coli. We are able to identify active prophage elements through the requirement for their repressors. We also find distinct differences in requirements for genes involved in cell surface struc

SUBMITTER: Barquist L 

PROVIDER: S-EPMC3632133 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature