Proteomic changes in cerebrospinal fluid of presymptomatic and affected persons carrying familial Alzheimer disease mutations.

ABSTRACT:

Objective

To identify cerebrospinal fluid (CSF) protein changes in persons who will develop familial Alzheimer disease (FAD) due to PSEN1 and APP mutations, using unbiased proteomics.

Design

We compared proteomic profiles of CSF from individuals with FAD who were mutation carriers (MCs) and related noncarriers (NCs). Abundant proteins were depleted and samples were analyzed using liquid chromatography-electrospray ionization-mass spectrometry on a high-resolution time-of-flight instrument. Tryptic peptides were identified by tandem mass spectrometry. Proteins differing in concentration between the MCs and NCs were identified.

Setting

A tertiary dementia referral center and a proteomic biomarker discovery laboratory.

Participants

Fourteen FAD MCs (mean age, 34.2

SUBMITTER: Ringman JM 

PROVIDER: S-EPMC3632731 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature