Automated operation of immiscible filtration assisted by surface tension (IFAST) arrays for streamlined analyte isolation.

ABSTRACT: The purification of analytes is an important prerequisite for many analytical processes. Although automated infrastructure has dramatically increased throughput for many of these processes, the upstream analyte purification throughput has lagged behind, partially due to the complexity of conventional isolation processes. Here, we demonstrate automated operation of arrays of a new sample preparation technology--immiscible filtration assisted by surface tension (IFAST). IFAST uses surface tension to position an immiscible liquid barrier between a biological sample and downstream buffer. Paramagnetic particles are used to capture analytes of interest and draw them across the immiscible barrier, thus resulting in purification in a single step. Furthermore, the planarity of the IFAST design ena

SUBMITTER: Berry SM 

PROVIDER: S-EPMC3633642 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature