Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system.

ABSTRACT: Toll-like receptors (TLRs) are an important class of pattern recognition receptors (PRRs) that recognize microbial and danger signals. Their downstream signaling upon ligand binding is vital for initiation of the innate immune response. In human and mammalian models, myeloid differentiation factor 88 (MYD88) is known for its central role as an adaptor molecule in interleukin 1 receptor (IL-1R) and TLR signaling. The zebrafish is increasingly used as a complementary model system for disease research and drug screening. Here, we describe a zebrafish line with a truncated version of MyD88 as the first zebrafish mutant for a TLR signaling component. We show that this immune-compromised mutant has a lower survival rate under standard rearing conditions and is more susceptible to challenge with

SUBMITTER: van der Vaart M 

PROVIDER: S-EPMC3634667 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature