Tissue homeostasis in the wing disc of Drosophila melanogaster: immediate response to massive damage during development.

ABSTRACT: All organisms have developed mechanisms to respond to organ or tissue damage that may appear during development or during the adult life. This process of regeneration is a major long-standing problem in Developmental Biology. We are using the Drosophila melanogaster wing imaginal disc to study the response to major damage inflicted during development. Using the Gal4/UAS/Gal80(TS) conditional system, we have induced massive cell killing by forcing activity of the pro-apoptotic gene hid in two major regions of the disc as defined by Gal4 inserts in the genes rotund (rn) and spalt (sal). The procedure ensures that at the end of a 40-48 hrs of ablation period the great majority of the cells of the original Rn or Sal domains have been eliminated. The results indicate that the damage provokes an

SUBMITTER: Herrera SC 

PROVIDER: S-EPMC3636033 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature