Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling.

ABSTRACT:

Background

Heterotrimeric guanine nucleotide binding proteins of the G12/13 subfamily, which includes the α-subunits Gα12 and Gα13, stimulate the monomeric G protein RhoA through interaction with a distinct subset of Rho-specific guanine nucleotide exchange factors (RhoGEFs). The structural features that mediate interaction between Gα13 and RhoGEFs have been examined in crystallographic studies of the purified complex, whereas a Gα12:RhoGEF complex has not been reported. Several signaling responses and effector interactions appear unique to Gα12 or Gα13, despite their similarity in amino acid sequence.

Methods

To comprehensively examine Gα12 for regions involved in RhoGEF interaction, we screened a panel of Gα12 cassette substitution mutants for binding to leukemia-associate

SUBMITTER: Ritchie BJ 

PROVIDER: S-EPMC3636079 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature