Metagenomic analysis of a tropical composting operation at the sao paulo zoo park reveals diversity of biomass degradation functions and organisms.

ABSTRACT: Composting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the São Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. The phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo co

SUBMITTER: Martins LF 

PROVIDER: S-EPMC3637033 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature