Lessons from high-throughput protein crystallization screening: 10 years of practical experience.

ABSTRACT:

Introduction

X-ray crystallography provides the majority of our structural biological knowledge at a molecular level and, in terms of pharmaceutical design, is a valuable tool to accelerate discovery. It is the premier technique in the field, but its usefulness is significantly limited by the need to grow well-diffracting crystals. It is for this reason that high-throughput crystallization has become a key technology that has matured over the past 10 years through the field of structural genomics. Areas covered : The authors describe their experiences in high-throughput crystallization screening in the context of structural genomics and the general biomedical community. They focus on the lessons learnt from the operation of a high-throughput crystallization-screening labor

SUBMITTER: Luft JR 

PROVIDER: S-EPMC3637395 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature