Maintenance of "stem cell" features of cartilage cell sub-populations during in vitro propagation.

ABSTRACT:

Background

The discovery of mesenchymal stem cells (MSCs) or MSC-like cells in cartilage tissue does not tie in well with the established view that MSCs derive from a perivascular niche. The presence of MSCs may raise concerns about specificity and application safety, particularly in terms of the regulatory site. The aim of the present study was to investigate the benefits or possible risks of the MSC-like properties of cells isolated from cartilage in the context of autologous chondrocyte implantation.

Methods

Chondrocytic cells were isolated from cartilage or intervertebral disc tissue. Flow cytometry was used to analyze the expression of cell surface antigens. MSC-like cells were either enriched or depleted by means of magnetic cell sorting (MACS) involving the monoclonal

SUBMITTER: Benz K 

PROVIDER: S-EPMC3637487 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature