Comparative proteomics study on liver mitochondria of primary biliary cirrhosis mouse model.

ABSTRACT:

Background

Primary biliary cirrhosis (PBC) is a liver specific chronic disease with unclear pathogenesis, especially for the early stage molecular events. The mitochondrion is a multi-functional organelle associated with various diseases including PBC. The purpose of this study was to discover the alterations in the mitochondria proteome using an early stage PBC mouse model for revealing the possible pathogenesis mechanisms in the early stages of PBC.

Methods

Mouse model of early stage of PBC was constructed by consecutive administration of poly I:C. Mitochondria of mouse models and controls were purified and comparative proteomics was performed by iTRAQ technology. Then, differentially expressed proteins were validated by western blotting.

Results

In total 354 protei

SUBMITTER: Song G 

PROVIDER: S-EPMC3637517 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature