Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria.

ABSTRACT:

Background

The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that can overcome the plasma membrane barrier and enter cells directly, either alone or with associated cargoes. However, the cellular entry mechanisms of CPPs in cyanobacteria have not been studied.

Results

In the present study, we determine CPP-mediated transduction efficiency and internalization mechanisms in cyanobacteria using a combination of biological and biophysical methods. We demonstrate that both Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC

SUBMITTER: Liu BR 

PROVIDER: S-EPMC3637573 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature