Prediction of constitutive A-to-I editing sites from human transcriptomes in the absence of genomic sequences.

ABSTRACT:

Background

Adenosine-to-inosine (A-to-I) RNA editing is recognized as a cellular mechanism for generating both RNA and protein diversity. Inosine base pairs with cytidine during reverse transcription and therefore appears as guanosine during sequencing of cDNA. Current approaches of RNA editing identification largely depend on the comparison between transcriptomes and genomic DNA (gDNA) sequencing datasets from the same individuals, and it has been challenging to identify editing candidates from transcriptomes in the absence of gDNA information.

Results

We have developed a new strategy to accurately predict constitutive RNA editing sites from publicly available human RNA-seq datasets in the absence of relevant genomic sequences. Our approach establishes new parameters to inc

SUBMITTER: Zhu S 

PROVIDER: S-EPMC3637798 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature