Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting.

ABSTRACT:

Background

Targeted capture, combined with massively-parallel sequencing, is a powerful technique that allows investigation of specific portions of the genome for less cost than whole genome sequencing. Several methods have been developed, and improvements have resulted in commercial products targeting the human or mouse exonic regions (the exome). In some cases it is desirable to custom-target other regions of the genome, either to reduce the amount of sequence that is targeted or to capture regions that are not targeted by commercial kits. It is important to understand the advantages, limitations, and complexity of a given capture method before embarking on a targeted sequencing experiment.

Results

We compared two custom targeted capture methods suitable for single chromos

SUBMITTER: Teer JK 

PROVIDER: S-EPMC3637801 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature