A zebrafish high throughput screening system used for Staphylococcus epidermidis infection marker discovery.

ABSTRACT:

Background

Staphylococcus epidermidis bacteria are a major cause of biomaterial-associated infections in modern medicine. Yet there is little known about the host responses against this normally innocent bacterium in the context of infection of biomaterials. In order to better understand the factors involved in this process, a whole animal model with high throughput screening possibilities and markers for studying the host response to S. epidermidis infection are required.

Results

We have used a zebrafish yolk injection system to study bacterial proliferation and the host response in a time course experiment of S. epidermidis infection. By combining an automated microinjection system with complex object parametric analysis and sorting (COPAS) technology we have quantified ba

SUBMITTER: Veneman WJ 

PROVIDER: S-EPMC3638012 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature