Divergent protein motifs direct elongation factor P-mediated translational regulation in Salmonella enterica and Escherichia coli.

ABSTRACT:

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Elongation factor P (EF-P) is a universally conserved bacterial translation factor homologous to eukaryotic/archaeal initiation factor 5A. In Salmonella, deletion of the efp gene results in pleiotropic phenotypes, including increased susceptibility to numerous cellular stressors. Only a limited number of proteins are affected by the loss of EF-P, and it has recently been determined that EF-P plays a critical role in rescuing ribosomes stalled at PPP and PPG peptide sequences. Here we present an unbiased in vivo investigation of the specific targets of EF-P by employing stable isotope labeling of amino acids in cell culture (SILAC) to compare the proteomes of wild-type and efp mutant Salmonella. We found that metabolic and motility genes are prominent among the subset of

SUBMITTER: Hersch SJ 

PROVIDER: S-EPMC3638311 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature