Detection of Pneumocystis in the nasal swabs of immune-suppressed rats by use of PCR and microscopy.

ABSTRACT:

Background

Detection of Pneumocystis jiroveci colonization in lungs or oral samples due to high sensitivity of PCR methods results in undue treatment of patients without any symptoms of Pneumocystis pneumonia. The aim of the present study is to demonstrate Pneumocystis carinii in rats, immune suppressed by oral and subcutaneous administration of dexamethasone.

Material/methods

Blood, oral, nasal and eye swabs were collected prior to immune suppression and 2, 6, 12 weeks after administration of dexamethasone. Also, samples were collected from lung, heart, liver, kidney, diaphragm, brain, spleen, tongue, muscle, eye, intestine, and feces. Cysts and trophozoites were investigated in stained slides and MSG gene was detected by PCR.

Results

The results showed that weight l

SUBMITTER: Can H 

PROVIDER: S-EPMC3638709 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature