Determination of ATP and ADP Secretion from Human and Mouse Platelets by an HPLC Assay.

ABSTRACT:

Background

Secretion of ADP and ATP is an essential prerequisite for platelet aggregation. Impaired nucleotide secretion can cause aggregation defects and increased bleeding risk. Quantitative determination of platelet nucleotide content and exocytosis is thus of importance for the characterization and diagnosis of bleeding phenotypes. For transgenic animal models with hemostatic defects analysis of potential secretion defects is as well imperative.

Methods

Supernatants of washed platelets and platelet-rich plasma were analyzed by HPLC for ADP and ATP concentration. Calibration of the HPLC data was accomplished with an internal standard compensating for loss of analyte, detection sensitivity, and interference of the biomatrix.

Results

HPLC analysis of nucleotide secre

SUBMITTER: von Papen M 

PROVIDER: S-EPMC3638993 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature