A serine/threonine phosphatase encoded by MG_207 of Mycoplasma genitalium is critical for its virulence.

ABSTRACT:

Background

Bacterial signal transduction systems like two component system (TCS) and Serine/Threonine kinase (STK) and Serine/Threonine phosphatase (STP) play important roles in the virulence and pathogenesis of bacterial pathogens. Mycoplasma genitalium, a mollicute that causes the urogenital diseases urethritis and cervicitis in men and women, respectively, is a pathogen which lacks TCS but possesses STK/STP. In this study, we investigated the biochemical and virulence properties of an STP protein encoded by the gene MG_207 of this species.

Results

We overexpressed MG207 in Escherichia coli overexpression system as a recombinant His10MG207 protein and purified it with affinity chromatography. This recombinant protein readily hydrolyzed the substrate p-nitrophenyl phosphate

SUBMITTER: Martinez MA 

PROVIDER: S-EPMC3639085 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature