Dynamic recruitment of microRNAs to their mRNA targets in the regenerating liver.

ABSTRACT:

Background

Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs.

Results

miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold re

SUBMITTER: Schug J 

PROVIDER: S-EPMC3639193 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature