Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton.

ABSTRACT:

Background

Genetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. A deep sequencing approach was used to investigate the global expression and complexity of small RNAs during cotton anther development in this study.

Results

Three small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton, resulting in nearly 80 million sequence reads. The total number of miRNAs and short interfering RNA

SUBMITTER: Wei M 

PROVIDER: S-EPMC3639194 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature