Transcriptional profiling of Candida glabrata during phagocytosis by neutrophils and in the infected mouse spleen.

ABSTRACT: Expression microarray analysis of Candida glabrata following phagocytosis by human neutrophils was performed, and results were compared with those from C. glabrata incubated under conditions of carbohydrate or nitrogen deprivation. Twenty genes were selected to represent the major cell processes altered by phagocytosis or nutrient deprivation. Quantitative real-time PCR (qRT-PCR) with TaqMan chemistry was used to assess expression of the same genes in spleens of mice infected intravenously with Candida glabrata. The results in spleen closely paralleled gene expression in neutrophils or following carbohydrate deprivation. Fungal cells responded by upregulating alternative energy sources through gluconeogenesis, glyoxylate cycle, and long-chain fatty acid metabolism. Autophagy was likely emp

SUBMITTER: Fukuda Y 

PROVIDER: S-EPMC3639592 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature