Development of an optimized medium, strain and high-throughput culturing methods for Methylobacterium extorquens.

ABSTRACT: Methylobacterium extorquens strains are the best-studied methylotrophic model system, and their metabolism of single carbon compounds has been studied for over 50 years. Here we develop a new system for high-throughput batch culture of M. extorquens in microtiter plates by jointly optimizing the properties of the organism, the growth media and the culturing system. After removing cellulose synthase genes in M. extorquens strains AM1 and PA1 to prevent biofilm formation, we found that currently available lab automation equipment, integrated and managed by open source software, makes possible reliable estimates of the exponential growth rate. Using this system, we developed an optimized growth medium for M. extorquens using response surface methodologies. We found that media that used EDTA a

SUBMITTER: Delaney NF 

PROVIDER: S-EPMC3639900 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature