An image-based biosensor assay strategy to screen for modulators of the microRNA 21 biogenesis pathway.

ABSTRACT: microRNAs (miRNAs) are evolutionary conserved, small endogenous non-coding, RNA molecules. Although their mode of action has been extensively studied, little is known about their biogenesis. As their altered expression has been implicated in many diseases, small molecules that would modulate their expression are sought after. They are generated through the concerted action of several complexes which promote their transcription, maturation, export, trafficking, and loading of mature miRNA into silencing complexes. An increasing number of studies have suggested that each of these steps serves as a regulatory junction in the process, and therefore provides an intervention point. For this purpose, we have developed a simple image-based assay strategy to screen for such modulators. Here, we des

SUBMITTER: Shum D 

PROVIDER: S-EPMC3640486 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature