Lumbar spine intervertebral disc gene delivery: a pilot study in lewis rats.

ABSTRACT:

Background

Basic research toward understanding and treating disc pathology in the spine has utilized numerous animal models, with delivery of small molecules, purified factors, and genes of interest. To date, gene delivery to the rat lumbar spine has only been described utilizing genetically programmed cells in a matrix which has required partial disc excision, and expected limitation of treatment diffusion into the disc.

Purpose

This study was designed to develop and describe a surgical technique for lumbar spine exposure and disc space preparation, and use of a matrix-free method for gene delivery.

Methods

Naïve or genetically programmed isogeneic bone marrow stromal cells were surgically delivered to adolescent male Lewis rat lumbar discs, and utilizing quantitativ

SUBMITTER: Damle SR 

PROVIDER: S-EPMC3640714 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature